What is blood?

What are the components of blood?

Blood is an opaque red coloured fluid that flows freely but is denser and more viscous than water.

Blood is made up of liquids and solids. The liquid part is called plasma and is made up of water, salts, and protein. A substantial part of blood contains RBCs, WBCs, and platelets.

Plasma: Carriers nutrients, hormones, and proteins to different parts of the body. About 55% of the blood consists of plasma.

RBCs: Delivers oxygen from lungs to tissues and organs.

WBCs: Fights infection and are a part of your immune system.

Platelets: Helps blood to clot when you have a cut or wound.

The other 45% of blood is made up of blood cells.

What do you mean by blood processing?

After blood donation from healthy or non-healthy patients for study or research purposes, the blood is separated into its components for further analysis. This is known as blood processing and can be achieved using the centrifugation technique.

Separating Plasma from Whole Blood

Steps:

  1. Draw 12 mL of Whole Blood for each 5 mL of plasma.
  2. Centrifuge for at least 15 minutes at 2200–2500 rpm.
  3. Pipette out plasma into a clean plastic screw-cap vial.

What is Plasma used for?

Separating Serum from Whole Blood

Steps:

  1. Invert the tube gently no more than eight times. Further inversion may cause alterations in sample integrity.
  2. Do not remove the stopper at any time. Do not centrifuge immediately after drawing blood. Allow the blood to clot in an upright position for at least 30 minutes but not longer than 1 hour before centrifugation.
  3. Centrifuge for at least 15 minutes at 2200–2500 rpm within one hour of collection.
  4. Transfer the serum to a plastic screw-cap vial for transport to the laboratory.

What is Serum used for?

Just getting started. . .